What are the effects of sunlight on phototropism and chlorophyll development of mongo seedlings?
What are the responses of young mongo seedlings to sunlight?
I predict that sunlight affects phototropism and chlorophyll development of mongo seedlings. Mongo seedlings will grow to the direction where light can be detected and will also develop chlorophyll at different levels depending on the amount of available light.
Scope and Limitation
This experiment is conducted and limited to the effects of sunlight on phototropism and chlorophyll development of green varieties of mongo seedlings.
Independent Variable: amount and direction of sunlight
Dependent Variable: growth direction and chlorophyll development of the mongo seedlings
Controlled Variable: Testing procedures, type of mongo seedlings, time plants grow & containers
To measure the dependent variable I will use a protractor to calculate the angle of seedlings’ growth direction and paper chromatography or Adobe Photoshop to quantify the green color of mongo seedlings.
Positive Control Setup: mongo seedlings fully exposed to sunlight
Negative Control Setup: mongo seedlings not exposed to sunlight
Experimental Setup: mongo seedlings partly exposed to only one direction of sunlight
30 pieces green mongo seeds, soaked overnight in tap water
48 ply 4” x 4”tissue paper damped in tap water
3 pieces empty plastic container with cover
1. Moisten each 16 ply of tissue paper with tap water then place it into the cover of each plastic container. Remove excess water that may pool around the lid.
2. Distribute evenly 10 pieces of soaked mongo seeds around on each moistened paper placed in the cover of the container.
3. Prepare three plastic containers according to the following different specifications:
a. Container A – as it is
b. Container B – covered with aluminum foil
c. Container C – covered with aluminum foil but with a window slit of 1 cm x 3 cm
4. Cover the prepared mongo seeds using the prepared plastic containers.
5. Make three (3) replicates of the three setups and label it properly.
6. Place the setups near a window. Arrange the slit so that it points towards the source of sunlight.
7. Leave the mongo seedlings to grow for five days. Don’t let the seedlings dry out by moistening the paper pads every day.
8. Make a daily observation for five (5) days by measuring the length and angle of the seedlings. Then take pictures to measure the green levels of each setup.
9. To get the length, use a string and run it along the sides of the mongo seedlings’ sprout. Then using a ruler, measure the length of the string.
10. To get the green levels of the seedlings, take photos of each setup and use Photoshop.
Results and Discussions